Establishment and characterization of a cisplatin-resistant cell line, KB-R, derived from oral carcinoma cell line, KB
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Establishment and characterization of a cisplatin-resistant cell line, KB-R, derived from oral carcinoma cell line, KB.
To investigate the mechanism of the resistance to cisplatin (CDDP), we established the CDDP-resistant cell line, KB-R, from CDDP-sensitive oral carcinoma cell line, KB. The 3-(3, 4-dimethyl-thiazol-2-yl) 2, 5-diphenyltetrazolium bromide (MTT) assay indicated that KB-R is 5.5-fold more resistant to CDDP than KB. Microarray analysis indicated that the expression levels of 1,718 genes were elevate...
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Introduction: In recent years, bismuth-based nanomaterials have been widely used in medical researches as imaging, drug delivery and x-ray radiosensitizing agents. Due to their anti-microbial effects against Helicobacter pylori (HP), bismuth colloidal compounds are used to treat various types of diseases such as chronic gastritis. Despite their advantages, bismuth-based compoun...
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proteins. The Ml subunit is a Mr 170,000 protein with a binding site for substrates such as CDP (4, 5). M2 (Mr 88,000) contains a tyrosyl free radical, a unique protein-bound organic free radical, and non heme iron. The iron and the tyrosyl groups are essential for the RR enzyme activity. HU is known to target on M2, interfering with the free radical of M2 and thereby inactivating RR activity (...
متن کاملCharacterization of a hydroxyurea-resistant human KB cell line with supersensitivity to 6-thioguanine.
Hydroxyurea (HU) is currently used in the clinic for the treatment of chronic myelogenous leukemia, head and neck carcinoma, and sarcoma. One of its drawbacks, however, is the development of HU resistance. To study this problem, we developed a HU-resistant human KB cell line which exhibits a 15-fold resistance to HU. The characterization of this HU-resistant phenotype revealed a gene amplificat...
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Tumor cells of a human medullary thyroid carcinoma were isolated and propagated in tissue culture. Several cell lines with different morphology developed from the primary culture, among others a fibroblast-like growing cell line (MTC-F) and a cell line growing as a suspension of single cells and spherical cell clusters (MTC-SK). The MTC-SK cell line was serially propagated for 90 passages, over...
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ژورنال
عنوان ژورنال: International Journal of Oncology
سال: 2007
ISSN: 1019-6439,1791-2423
DOI: 10.3892/ijo.30.6.1325